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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc

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Kit-based synthesis of 2-deoxy-2-[<sup>18</sup>F]-fluoro-<span class="small-caps u-small-caps">d</span>-sorbitol for bacterial imaging

Nature Protocols, Published online: 22 October 2021; doi:10.1038/s41596-021-00613-2Mota et al. describe a protocol for the rapid, room-temperature, kit-based synthesis of 18F-FDS for positron emission tomography imaging.

Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells

Nature Protocols, Published online: 22 October 2021; doi:10.1038/s41596-021-00614-1Split-GFP-based contact site sensors (SPLICS) are capable of reconstituting fluorescence when two opposing membranes come into close proximity. SPLICS can be used to quantify membrane contact sites between organelles in both fixed and living cells.

Kit-based synthesis of 2-deoxy-2-[<sup>18</sup>F]-fluoro-<span class="small-caps u-small-caps">d</span>-sorbitol for bacterial imaging

Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Nature Protocols, Published online: 18 October 2021; doi:10.1038/s41596-021-00608-zThis protocol provides a step-by-step workflow for ATAC-Me, an integrated method for simultaneously probing DNA methylation and chromatin accessibility from a single DNA fragment library.

Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

Nature Protocols, Published online: 18 October 2021; doi:10.1038/s41596-021-00607-0The polarity of gastrointestinal organoids is reversed to study epithelial biology and host–microbe interactions. Access to the apical surface of the epithelium is increased while preserving epithelial integrity and secretory and absorptive functions.

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

Nature Protocols, Published online: 14 October 2021; doi:10.1038/s41596-021-00507-3This protocol describes a scalable approach for joint profiling of chromatin accessibility and gene expression in single cells or nuclei that relies on multiple rounds of combinatorial indexing and can identify cell-specific regulatory elements.

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

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