Nature Protocols- Issue - nature.com science feeds
Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00974-4Isotopically labeled amino acids are useful in pharmacology and for medical imaging. In this protocol, C1-labeled α-amino acids are prepared via late-stage carboxylate exchange of unprotected α-amino acids with [*C]CO2 where *C is 13C, 11C or 14C.
Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00976-2We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.
Nature Protocols, Published online: 26 March 2024; doi:10.1038/s41596-024-00971-7This protocol can be used to generate three-dimensional vascularized bone marrow organoids from human induced pluripotent stem cells. The organoids contain key stromal and hematopoietic cell types and can be engrafted with normal and malignant cells from adult donors to...
Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00966-4This protocol is for using PanSyn, the first software package for the identification of micro- and macrosynteny and their functional integration for comprehensive characterization of genome architecture and regulatory evolution.
Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00970-8Cell engineering using polymeric material is an area that remains largely unexplored. This protocol describes two light-driven approaches for synthesizing bioactive polymers within intricate intracellular settings.
Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00954-8Genetic interactions have been found to influence phenotypes in a variety of systems, yet their specific contribution to complex diseases remains unclear. This protocol describes Bridging Gene sets with Epistasis (BridGE), a computational approach for discovering interactions...
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