Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS)

Nature Protocols, Published online: 10 July 2020; doi:10.1038/s41596-020-0344-2This protocol describes procedures for isolating the protein-bound transcriptome and RNA-binding proteome via UV crosslinking and organic/aqueous phase separation.

3h

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

Nature Protocols, Published online: 10 July 2020; doi:10.1038/s41596-020-0339-zThis protocol offers a CRISPR-based toolkit, including several variants of ‘classic’ CRISPR–Cas9, along with CRISPRi and CRISPR-base editing systems (CRISPR-BEST) for genome editing in streptomycetes.

3h

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

4h

Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS)

4h

lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements

Nature Protocols, Published online: 08 July 2020; doi:10.1038/s41596-020-0333-5This protocol describes a lentivirus-based massively parallel reporter assay (lentiMPRA) for quantitative assessment of the activity of gene regulatory elements and an accompanying Nextflow-based computational pipeline for analysis.

2d

lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements

2d

A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents

Nature Protocols, Published online: 06 July 2020; doi:10.1038/s41596-020-0324-6Pupil diameter is measured in rodents using low-cost cameras and a Raspberry Pi computer to obtain a rapid, noninvasive real-time readout of locus coeruleus activity.

4d

Geometric morphometrics of microscopic animals as exemplified by model nematodes

Nature Protocols, Published online: 06 July 2020; doi:10.1038/s41596-020-0347-zThis protocol describes how to perform geometric morphometrics on microscopic animals, as exemplified by analysis of nematode mouth morphology. The procedure provides guidance for microscopy data acquisition, data analysis and validation.

4d

Geometric morphometrics of microscopic animals as exemplified by model nematodes

4d

A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents

4d