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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc

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Facile synthesis of per(6-<i>O</i>-<i>tert</i>-butyldimethylsilyl)-α-, β-, and γ-cyclodextrin as protected intermediates for the functionalization of the secondary face of the macrocycles

Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos

Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes

Recombinant extracellular vesicles as biological reference material for method development, data normalization and assessment of (pre-)analytical variables

Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos

Nature Protocols, Published online: 15 January 2021; doi:10.1038/s41596-020-00449-2This protocol describes how to perform combined protein isoform detection with nucleic acid analysis from the same individual mouse embryo or blastomere using fractionation polyacrylamide gel electrophoresis (fPAGE).

Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes

Nature Protocols, Published online: 15 January 2021; doi:10.1038/s41596-020-00457-2A protocol for implementing Brillouin microscopy to study biological materials. The procedure contains instructions for integrating an add-on Brillouin module with an existing confocal microscope as well as for its calibration and use in data collection and processing.

Facile synthesis of per(6-<i>O</i>-<i>tert</i>-butyldimethylsilyl)-α-, β-, and γ-cyclodextrin as protected intermediates for the functionalization of the secondary face of the macrocycles

Nature Protocols, Published online: 15 January 2021; doi:10.1038/s41596-020-00443-8Reversible protection of the primary face of cyclodextrins by silylation is a very popular strategy for modification of the secondary rim. This protocol describes how to prepare these important intermediates in high yield and purity.

Recombinant extracellular vesicles as biological reference material for method development, data normalization and assessment of (pre-)analytical variables

Nature Protocols, Published online: 15 January 2021; doi:10.1038/s41596-020-00446-5This Protocol Extension presents recombinant extracellular vesicles as reference materials for method development and standardization. It details their characterization and detection in spiked samples by fluorescence, nucleic acid and protein measurements.

Author Correction: A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents

Author Correction: A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents

Nature Protocols, Published online: 14 January 2021; doi:10.1038/s41596-021-00493-6Author Correction: A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents

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