Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc
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Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials

Nature Protocols, Published online: 11 April 2024; doi:10.1038/s41596-024-00983-3Activated neutrophils labeled with NIR-II lanthanide downshifting nanoparticles can be sequentially imaged through the intact skull of a mouse model of ischemic stroke during adhesion, crawling and extravasation processes

Thu Apr 11, 2024 21:10
Control of DNA replication in vitro using a reversible replication barrier

Nature Protocols, Published online: 09 April 2024; doi:10.1038/s41596-024-00977-1This protocol describes the establishment of a reversible replication barrier using plasmid templates containing a lacO array bound by LacR repressor. The method allows fine control of replication fork movement and replication fork encounter with DNA lesions.

Tue Apr 9, 2024 18:10
Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

Nature Protocols, Published online: 04 April 2024; doi:10.1038/s41596-024-00973-5It can be challenging to obtain meaningful and accurate structural information for air-sensitive proteins. This protocol describes the application of customized vacuum manifold and anaerobic chamber setups for the purification and cryo-electron microscopy analysis of air-sensitive...

Thu Apr 4, 2024 21:09
Packaging and containerization of computational methods

Nature Protocols, Published online: 02 April 2024; doi:10.1038/s41596-024-00986-0Attempts to reproduce the computational steps described in published omics research often fail. This review provides guidelines for the packaging and containerization of software so that readers can use the exact programs used in published work.

Tue Apr 2, 2024 21:13
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO<sub>2</sub>

Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00974-4Isotopically labeled amino acids are useful in pharmacology and for medical imaging. In this protocol, C1-labeled α-amino acids are prepared via late-stage carboxylate exchange of unprotected α-amino acids with [*C]CO2 where *C is 13C, 11C or 14C.

Thu Mar 28, 2024 16:39
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks

Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00976-2We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.

Thu Mar 28, 2024 16:39

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