Nature Protocols, Published online: 25 July 2024; doi:10.1038/s41596-024-01025-8This protocol describes the in vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis. Given the ethical limitations restricting the use of human embryos beyond 14 days, nonhuman primate embryos are an ideal model.

Nature Protocols, Published online: 23 July 2024; doi:10.1038/s41596-024-01019-6This protocol adds a surface nanocoating to bacteria to improve bioavailability and reduce side effects for enhanced microbial therapy. Living bacterial therapeutics are produced by nanocoating bacteria individually via either chemical decoration or physical encapsulation.

Nature Protocols, Published online: 23 July 2024; doi:10.1038/s41596-024-01009-8Engineered and anthropogenic nanoscale materials in the environment acquire a coating of biomolecules (biocorona) that modulates their properties, uptake and biodistribution. This protocol streamlines biocorona analysis to support the development of safe and sustainable...

Nature Protocols, Published online: 22 July 2024; doi:10.1038/s41596-024-01021-yFIND-seq is a nucleic acid cytometry platform capable of isolating rare cells on the basis of RNA or DNA markers. This protocol outlines the molecular and microfluidic steps to perform FIND-seq, followed by bulk or single-cell transcriptomic analysis.

Nature Protocols, Published online: 18 July 2024; doi:10.1038/s41596-024-01022-xThe authors present MARVE, a paper-based, preamplification-free diagnostic tool for fast but accurate colorimetric detection of viral variants at single-nucleotide resolution.

Nature Protocols, Published online: 18 July 2024; doi:10.1038/s41596-024-01017-8Complex carbohydrates that are not broken down by human enzymes are food sources for gut microbiota. Toward understanding this process, this protocol describes the quantitative analysis of carbohydrates in food and fecal samples by using LC-MS/MS.