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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc

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A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Nature Protocols, Published online: 13 May 2022; doi:10.1038/s41596-022-00694-7This protocol details the synthesis and use of Peroxymycin-1, an activity-based histochemical probe for hydrogen peroxide detection in fixed cell and mouse tissue samples.

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Nature Protocols, Published online: 11 May 2022; doi:10.1038/s41596-022-00693-8In this work, ‘hyperpolarized water’ produced by cryogenic dynamic nuclear polarization is used to boost signal intensities in NMR of proteins and nucleic acids. This allows NMR at lower concentrations and detection of folding intermediates.

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection

Nature Protocols, Published online: 11 May 2022; doi:10.1038/s41596-022-00695-6Mouse or human organoids are generated from two cervical regions: columnar epithelium-lined endocervix and stratified squamous epithelium-lined ectocervix. Genetic manipulation for modeling cancers and organoid infections is also described.

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Chromatin accessibility profiling by ATAC-seq

Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00692-9A protocol for generating chromatin accessibility profiles from a broad variety of cell and tissue types, including a step-by-step workflow for library preparation and guidelines for data processing and downstream analysis.

All-optical interrogation of neural circuits in behaving mice

Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00691-wThis protocol describes all-optical interrogation experiments in awake, behaving mice, demonstrating the utility of this strategy in three brain areas—barrel cortex, visual cortex and hippocampus—by using different experimental setups.

Sister chromatid–sensitive Hi-C to map the conformation of replicated genomes

Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00687-6This protocol combines in situ chromosome conformation capture with labeling of nascent DNA with the synthetic nucleoside 4-thio-thymidine to generate genome-wide contact probability maps within and across sister chromatids in mammalian cells.

Chromatin accessibility profiling by ATAC-seq

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