Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00506-4Primary, nonmalignant, human GC B cells are purified and expanded in culture. Genetic manipulation and engraftment into mice enable the generation of genetically customized, synthetic models of human lymphoma.

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Identifying high-confidence capture Hi-C interactions using CHiCANE

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00498-1The capture Hi-C assay and its variants require specialized statistical methods for identification of high-confidence contacts. This protocol presents a versatile computational pipeline for detecting interactions and performing downstream analyses.

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Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00500-wHere, we present a protocol for high-throughput screening of SPOT-peptide arrays to assess the antibiofilm, antimicrobial and immunomodulatory activities of synthetic peptides.

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Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00499-0This Protocol Extension details the use of zebrafish larvae as a simple and robust in vivo system for studying human norovirus infection, enabling evaluation of the antiviral effects and toxicities of small molecules.

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A well plate–based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature

Nature Protocols, Published online: 31 March 2021; doi:10.1038/s41596-020-00490-1This protocol describes how to integrate organoids into a vascularized organ-on-a-chip biofabricated platform. The platform is assembled in a 96-well format and allows the connection of two tissues through a single endothelialized microchannel blood vessel.

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A well plate–based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature

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Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq

Nature Protocols, Published online: 26 March 2021; doi:10.1038/s41596-021-00501-9The N4-acetylcytidine RNA modification is conserved in all domains of life. Here, the authors provide a detailed protocol for whole-transcriptome quantitative nucleotide resolution mapping of this modified nucleobase in different organisms.

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Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Nature Protocols, Published online: 26 March 2021; doi:10.1038/s41596-021-00497-2Human induced pluripotent stem cells are differentiated to the three main cell types found in the heart—cardiomyocytes, cardiac fibroblasts and cardiac endothelial cells—and then combined to form 3D cardiac microtissues.

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Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

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Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq

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