Nature Protocols, Published online: 15 October 2024; doi:10.1038/s41596-024-01087-8Publisher Correction: Generation of ‘semi-guided’ cortical organoids with complex neural oscillations

Nature Protocols, Published online: 14 October 2024; doi:10.1038/s41596-024-01060-5We describe the use of ColabFold to perform structure prediction of monomers, complexes and alternative conformations, either on the web or locally, and provide guidance on interpreting the results through confidence metrics and visualizations.

Nature Protocols, Published online: 10 October 2024; doi:10.1038/s41596-024-01057-0Kallisto, bustools and kb-python are a set of tools for quantifying bulk, single-cell and single-nucleus RNA-seq. Together, this set of free, open-source software tools can produce gene expression quantification from raw sequencing reads.

Nature Protocols, Published online: 08 October 2024; doi:10.1038/s41596-024-01062-3Synthetic polypeptides are used in many applications in which biocompatibility is important. This protocol describes a fast lithium hexamethyldisilazide-initiated N-carboxyanhydride ring-opening polymerization method for their synthesis.

Nature Protocols, Published online: 04 October 2024; doi:10.1038/s41596-024-01055-2Channel-forming membrane proteins control solute exchange across membranes, and they are difficult to obtain with sufficient yield and purity. This protocol describes a universal approach to their expression (in Escherichia coli), extraction and purification.

Nature Protocols, Published online: 03 October 2024; doi:10.1038/s41596-024-01048-1An approach that combines a pH-sensitive synaptic biomarker expressed in vivo with the ex vivo staining of oligodendrocyte precursor cells enables quantification of synapse engulfment by oligodendrocyte precursor cells at single-cell and population-level resolution.