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Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc

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Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00506-4Primary, nonmalignant, human GC B cells are purified and expanded in culture. Genetic manipulation and engraftment into mice enable the generation of genetically customized, synthetic models of human lymphoma.

Identifying high-confidence capture Hi-C interactions using CHiCANE

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00498-1The capture Hi-C assay and its variants require specialized statistical methods for identification of high-confidence contacts. This protocol presents a versatile computational pipeline for detecting interactions and performing downstream analyses.

Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00500-wHere, we present a protocol for high-throughput screening of SPOT-peptide arrays to assess the antibiofilm, antimicrobial and immunomodulatory activities of synthetic peptides.

Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors

Nature Protocols, Published online: 09 April 2021; doi:10.1038/s41596-021-00499-0This Protocol Extension details the use of zebrafish larvae as a simple and robust in vivo system for studying human norovirus infection, enabling evaluation of the antiviral effects and toxicities of small molecules.

A well plate–based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature

Nature Protocols, Published online: 31 March 2021; doi:10.1038/s41596-020-00490-1This protocol describes how to integrate organoids into a vascularized organ-on-a-chip biofabricated platform. The platform is assembled in a 96-well format and allows the connection of two tissues through a single endothelialized microchannel blood vessel.

A well plate–based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature

Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq

Nature Protocols, Published online: 26 March 2021; doi:10.1038/s41596-021-00501-9The N4-acetylcytidine RNA modification is conserved in all domains of life. Here, the authors provide a detailed protocol for whole-transcriptome quantitative nucleotide resolution mapping of this modified nucleobase in different organisms.

Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Nature Protocols, Published online: 26 March 2021; doi:10.1038/s41596-021-00497-2Human induced pluripotent stem cells are differentiated to the three main cell types found in the heart—cardiomyocytes, cardiac fibroblasts and cardiac endothelial cells—and then combined to form 3D cardiac microtissues.

Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq

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