Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a free-to-access community site, 'Protocols Network', containing protocols that are posted direc
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A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO<sub>2</sub>

Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00974-4Isotopically labeled amino acids are useful in pharmacology and for medical imaging. In this protocol, C1-labeled α-amino acids are prepared via late-stage carboxylate exchange of unprotected α-amino acids with [*C]CO2 where *C is 13C, 11C or 14C.

Thu Mar 28, 2024 16:39
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks

Nature Protocols, Published online: 28 March 2024; doi:10.1038/s41596-024-00976-2We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.

Thu Mar 28, 2024 16:39
Generating human bone marrow organoids for disease modeling and drug discovery

Nature Protocols, Published online: 26 March 2024; doi:10.1038/s41596-024-00971-7This protocol can be used to generate three-dimensional vascularized bone marrow organoids from human induced pluripotent stem cells. The organoids contain key stromal and hematopoietic cell types and can be engrafted with normal and malignant cells from adult donors to...

Tue Mar 26, 2024 21:13
Pan-evolutionary and regulatory genome architecture delineated by an integrated macro- and microsynteny approach

Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00966-4This protocol is for using PanSyn, the first software package for the identification of micro- and macrosynteny and their functional integration for comprehensive characterization of genome architecture and regulatory evolution.

Fri Mar 22, 2024 00:14
Light-mediated intracellular polymerization

Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00970-8Cell engineering using polymeric material is an area that remains largely unexplored. This protocol describes two light-driven approaches for synthesizing bioactive polymers within intricate intracellular settings.

Thu Mar 21, 2024 19:41
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS

Nature Protocols, Published online: 21 March 2024; doi:10.1038/s41596-024-00954-8Genetic interactions have been found to influence phenotypes in a variety of systems, yet their specific contribution to complex diseases remains unclear. This protocol describes Bridging Gene sets with Epistasis (BridGE), a computational approach for discovering interactions...

Thu Mar 21, 2024 19:41

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