Large language models generate functional protein sequences across diverse families

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01618-2A generative deep-learning model designs artificial proteins with desired enzymatic activities.

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Spatial transcriptomics for profiling the tropism of viral vectors in tissues

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01648-wDual mapping shows localization patterns of AAV and RNA in intact tissues.

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CLASH enables large-scale parallel knock-in for cell engineering

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-023-01664-4CLASH is a CRISPR-based platform that enables the parallel knock-in via homology-directed repair of a large pool of transgene variants encoded in adeno-associated virus vectors. CLASH can be applied to the systematic and unbiased selection of favorable features in...

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Hallucinating functional protein sequences

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01634-2Functional proteins with limited homology to natural proteins are designed using a large language model.

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In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01558-xHuman intestinal organoids are endowed with immune cells by transplantation into humanized mice.

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Massively parallel knock-in engineering of human T cells

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01639-xUnbiased selection of CAR-T cells is achieved by massively parallel knock-in engineering.

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A universal deep-learning model for zinc finger design enables transcription factor reprogramming

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01624-4Zinc finger design is facilitated with a deep-learning model.

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Three-dimensional structured illumination microscopy with enhanced axial resolution

Nature Biotechnology, Published online: 26 January 2023; doi:10.1038/s41587-022-01651-1Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference.

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity

Nature Biotechnology, Published online: 24 January 2023; doi:10.1038/s41587-023-01665-3Targeted RNA degradation remains a challenge using existing methods owing to off-target effects and toxicity. We adapted the CRISPR-Csm complex, a multi-protein effector from type III CRISPR systems, for precise knockdown of nuclear or cytoplasmic transcripts in eukaryotic...

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Precise transcript targeting by CRISPR-Csm complexes

Nature Biotechnology, Published online: 23 January 2023; doi:10.1038/s41587-022-01649-9The bacterial Csm complex efficiently knocks down eukaryotic nuclear and cytoplasmic RNAs.

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