StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy

Nature Methods, Published online: 02 August 2021; doi:10.1038/s41592-021-01209-0StrucGP offers a de novo glycan mapping method to determine detailed N-glycan structures at the site-specific level.

12h

Transgenic mice for in vivo epigenome editing with CRISPR-based systems

Nature Methods, Published online: 02 August 2021; doi:10.1038/s41592-021-01207-2Two conditional transgenic mouse lines based on CRISPRa and CRISPRi enable epigenome editing in vivo.

12h

Spatial omics and multiplexed imaging to explore cancer biology

Nature Methods, Published online: 02 August 2021; doi:10.1038/s41592-021-01203-6This Review describes spatial omics and multiplexed imaging technologies and their current and future impact in studying tumor heterogeneity and cancer biology.

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Neural networks learn the motions of molecular machines

Nature Methods, Published online: 29 July 2021; doi:10.1038/s41592-021-01235-yNew computational approaches capture molecular motion from cryo-EM images and provide a more complete understanding of protein dynamics.

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Deep learning-based mixed-dimensional Gaussian mixture model for characterizing variability in cryo-EM

Nature Methods, Published online: 29 July 2021; doi:10.1038/s41592-021-01220-5e2gmm uses a deep neural network with a Gaussian representation to resolve the compositional and conformational variability within biomolecules using cryo-EM data.

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DOME: recommendations for supervised machine learning validation in biology

Nature Methods, Published online: 27 July 2021; doi:10.1038/s41592-021-01205-4DOME is a set of community-wide recommendations for reporting supervised machine learning–based analyses applied to biological studies. Broad adoption of these recommendations will help improve machine learning assessment and reproducibility.

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Graphical assessment of tests and classifiers

Nature Methods, Published online: 27 July 2021; doi:10.1038/s41592-021-01232-1I do not think you can start with anything precise. You have to achieve such precision as you can, as you go along. —Bertrand Russell

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The long and winding road of development: a coordinated song of transcription factors

Nature Methods, Published online: 26 July 2021; doi:10.1038/s41592-021-01219-yA four-dimensional single-cell atlas of transcription factor expression in Caenorhabditis elegans allows the identification of novel regulators of embryo development and the generation of molecular models of cell fate specification.

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A 4D single-cell protein atlas of transcription factors delineates spatiotemporal patterning during embryogenesis

Nature Methods, Published online: 26 July 2021; doi:10.1038/s41592-021-01216-1A protein expression atlas of transcription factors charted onto cell lineage maps of Caenorhabditis elegans development that uncovers mechanisms of spatiotemporal cell fate patterning and regulators of embryogenesis.

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Universal autofocus for quantitative volumetric microscopy of whole mouse brains

Nature Methods, Published online: 26 July 2021; doi:10.1038/s41592-021-01208-1RAPID (rapid autofocusing via pupil-split image phase detection) is a sample-agnostic real-time autofocus method for widefield microscopy. RAPID removes most image degradation in large, cleared samples for enhanced quantitative analyses.

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