Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences.
Nature Methods, Published online: 02 February 2023; doi:10.1038/s41592-023-01798-yAuthor Correction: Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein
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Nature Methods, Published online: 30 January 2023; doi:10.1038/s41592-022-01761-3LILAC is a photoactivatable version of Lifeact, a tool for labeling F-actin. LILAC can help avoid cytotoxicity, which is sometimes associated with the use of Lifeact.
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Nature Methods, Published online: 24 January 2023; doi:10.1038/s41592-022-01729-3Communication between cells is crucial for coordinated cellular functions in multicellular organisms. We present an optimal transport theory-based tool to infer cell–cell communication networks, spatial signaling directions and downstream targets in multicellular systems...
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Nature Methods, Published online: 23 January 2023; doi:10.1038/s41592-022-01741-7A deep learning approach called DeepPiCt facilitates segmentation and macromolecular identification in the cellular jungle of electron cryotomography data.
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Nature Methods, Published online: 23 January 2023; doi:10.1038/s41592-022-01746-2DeePiCt (deep picker in context) is a versatile, open-source deep-learning framework for supervised segmentation and localization of subcellular organelles and biomolecular complexes in cryo-electron tomography.
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Nature Methods, Published online: 23 January 2023; doi:10.1038/s41592-022-01728-4This work presents a computational framework, COMMOT, to spatially infer cell–cell communication from transcriptomics data based on a variant of optimal transport (OT).
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Nature Methods, Published online: 23 January 2023; doi:10.1038/s41592-023-01768-4Author Correction: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
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Nature Methods, Published online: 19 January 2023; doi:10.1038/s41592-023-01765-7Publisher Correction: Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
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Nature Methods, Published online: 19 January 2023; doi:10.1038/s41592-022-01753-3An optimized pipeline for improved inference and analysis of structural variants (SVs) has been developed, which uses Iris for refining breakpoints and sequences, and Jasmine for comparing SV calls at population scale.
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Nature Methods, Published online: 19 January 2023; doi:10.1038/s41592-022-01751-5Hybrid Unmixing offers enhanced imaging of multiplexed fluorescence labels, enabling longitudinal imaging of multiple fluorescent signals with reduced illumination intensities.
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