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Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences.

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Time for NanoNeuro

Nature Methods, Published online: 18 October 2021; doi:10.1038/s41592-021-01270-9This Perspective discusses how neuroscience can benefit from harnessing nanoscience-based tools for manipulating and recording neuronal activity.

MethodsJ2: a software tool to capture metadata and generate comprehensive microscopy methods text

Nature Methods, Published online: 15 October 2021; doi:10.1038/s41592-021-01290-5MethodsJ2: a software tool to capture metadata and generate comprehensive microscopy methods text

The class imbalance problem

Nature Methods, Published online: 15 October 2021; doi:10.1038/s41592-021-01302-4The exception proves the rule.

Removing independent noise in systems neuroscience data using DeepInterpolation

Nature Methods, Published online: 14 October 2021; doi:10.1038/s41592-021-01285-2DeepInterpolation is a self-supervised deep learning-based denoising approach for calcium imaging, electrophysiology and fMRI data. The approach increases the signal-to-noise ratio and allows extraction of more information from the processed data than from the raw data.

MDEmic: a metadata annotation tool to facilitate management of FAIR image data in the bioimaging community

Nature Methods, Published online: 11 October 2021; doi:10.1038/s41592-021-01288-zMDEmic: a metadata annotation tool to facilitate management of FAIR image data in the bioimaging community

Mapping genome structures in single cells

Nature Methods, Published online: 04 October 2021; doi:10.1038/s41592-021-01294-1Researchers develop single-cell SPRITE to detect higher-order 3D genome structures in single cells.

Keeping checks on machine learning

Nature Methods, Published online: 04 October 2021; doi:10.1038/s41592-021-01300-6Community-driven initiatives are proposing standards to improve the reporting and reproducibility of machine learning in biology. We support these developments, some of which are described in this month’s special issue.

Nanopore-detectable reporter proteins

Nature Methods, Published online: 04 October 2021; doi:10.1038/s41592-021-01297-yNanoporeTERs, engineered reporter proteins, can be detected on MinION nanopore sensor arrays.

Reprogrammed anti-tumor NK cells

Nature Methods, Published online: 04 October 2021; doi:10.1038/s41592-021-01296-zReprogrammed natural killer cells show enhanced functional properties and anti-tumor efficacy.

Spike inference with CASCADE

Nature Methods, Published online: 04 October 2021; doi:10.1038/s41592-021-01295-0A deep-learning-based tool and a large ground truth dataset enable spike inference from calcium imaging data acquired in a variety of experimental conditions.

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