Nature Methods, Published online: 30 April 2024; doi:10.1038/s41592-024-02262-1This Review summarizes the computational strategies for microbiome analysis using long-read sequencing data.

Nature Methods, Published online: 29 April 2024; doi:10.1038/s41592-024-02256-zCombining genome-wide CRISPR–Cas9-mediated base editors with temporally resolved phosphoproteomics enables the functional screening of thousands of post-translational modification sites involved in T cell activation.

Nature Methods, Published online: 25 April 2024; doi:10.1038/s41592-024-02247-0ESI-cryoPrep is a cryo-EM specimen preparation method that employs electrospray ionization techniques to deposit charged macromolecule-containing droplets on EM grids. Demonstrated across various protein samples, this approach effectively prevents biomolecule adsorption at...

Nature Methods, Published online: 24 April 2024; doi:10.1038/s41592-024-02242-5Super-resolution imaging of reference and target structures enables precise determination of the labeling efficiency of high-affinity binding proteins in cells for improved quantitative assessment of protein organization at the single-molecule level.

Nature Methods, Published online: 24 April 2024; doi:10.1038/s41592-024-02255-0SABR-IIs are engineered bifunctional receptors that enable CD4+ T cell antigen discovery.

Nature Methods, Published online: 23 April 2024; doi:10.1038/s41592-024-02263-0To those who seek transcriptomic information at high resolution, scale and throughput, single-cell RNA sequencing brings the data. Scientists share tips and future plans as they reflect on the method’s rise to stardom.