Hey, I know it's been 5 years but I have the same issue. When I run the test the software is unable to perform and always deliver the following result. The number of uniquely-identified sequences in spombe.fasta is 5142. The number of ePKs in spombe.fasta is 0: ePKs comprise 0.000 % of this proteome. The fraction of core ePK families found in this...

If they can't help you, we probably can't do much either. But we can optimize your STAR command. BAM SortedByCoordinate is the worst setting you can have for BAM output, switch to BAM Unsorted. You can always sort later using samtools. Also, set RAM consumption parameters explicitly.

Hi, you can check it from github here: [https://zwdzwd.github.io/InfiniumAnnotation][1]. I don't know if this is what you are looking for your Chip. In any case, there are pipelines and R package to do liftover , such as mLiftOver or ELMER to identify tumor-specific changes in DNA methylation. Hope this can help you Marco ...

Hi there, I am working on the de novo analysis of bacteria. This bacteria is related to Bacillus paranthracis, which was identified using rMLST, TYGS, and BLAST analysis. The genome assembly file was then provided to the KEGG-KASS tool. Although the prokaryotic dataset was selected for analysis, the identified KO terms seem irrelevant to bacteria,...

It makes absolutely no sense to conduct such an analysis. I strongly discourage you to even start with it. Results will be utterly nonsense for aforementioned reasons.

Cross-posted: https://support.bioconductor.org/p/9158194/