Nature Protocols, Published online: 26 April 2024; doi:10.1038/s41596-024-00975-3Ancient proteins carry genetic information from fossils that are too old or degraded for ancient DNA recovery. This protocol describes the extraction and tandem mass spectrometry sequencing of million-year-old dental enamel proteins for phylogenetic inference.

Nature Protocols, Published online: 25 April 2024; doi:10.1038/s41596-024-00978-0This protocol details methods for using methanol in methylation reactions, including the synthesis of suitable transition metal-containing catalysts, and in the synthesis of heterocycles. The methods described produce only H2 and H2O as by-products.

Nature Protocols, Published online: 23 April 2024; doi:10.1038/s41596-024-00981-5Isotope ratio mass spectrometry as described in this protocol can be used to determine natural variation in the abundance of stable isotopes in individual compounds to provide information relevant to metabolism, ecology or climate change.

Nature Protocols, Published online: 23 April 2024; doi:10.1038/s41596-024-00992-2Surface photovoltage microscopy as described in this protocol allows high spatial and energy resolution mapping of surface-charge distributions on photocatalyst particles, enabling rational design of improved materials.

Nature Protocols, Published online: 18 April 2024; doi:10.1038/s41596-024-00979-zNanoLuciferase- and HaloTag-based screening technologies are versatile tools suitable for the live-cell analysis of the entire small-molecule-induced degradation cascade to uncover the mode of action of proximity-inducing compounds such as PROTACs.

Nature Protocols, Published online: 18 April 2024; doi:10.1038/s41596-024-00990-4This protocol describes the preparation of long-lasting aggregation-induced emission-based, near-infrared afterglow luminescence nanoprobes. Their enhanced afterglow intensity results in improved imaging sensitivity and depth in vivo.