Nature Methods, Published online: 08 May 2024; doi:10.1038/s41592-024-02268-9Molecular Pixelation is an optics-free method that uses DNA-tagged antibodies to enable identification of the relative location of proteins on single cells.

Nature Methods, Published online: 07 May 2024; doi:10.1038/s41592-024-02296-5Author Correction: brainlife.io: a decentralized and open-source cloud platform to support neuroscience research

Nature Methods, Published online: 06 May 2024; doi:10.1038/s41592-024-02265-yCustom-built mathematical models make the immune response more predictable and offer mechanistic insights into fundamental immunology.

Nature Methods, Published online: 30 April 2024; doi:10.1038/s41592-024-02264-zCombining post-translational modification site-centric base editing with phenotypic screens uncovers the function of phosphorylation sites in high throughput, enabling the study of expansive signaling networks at a speed comparable to that of functional genomics.

Nature Methods, Published online: 30 April 2024; doi:10.1038/s41592-024-02262-1This Review summarizes the computational strategies for microbiome analysis using long-read sequencing data.

Nature Methods, Published online: 29 April 2024; doi:10.1038/s41592-024-02256-zCombining genome-wide CRISPR–Cas9-mediated base editors with temporally resolved phosphoproteomics enables the functional screening of thousands of post-translational modification sites involved in T cell activation.