During autophagy, double membrane autophagosomes engulf damaged organelles, protein aggregates, and pathogens. The cargo is initially sequestered by the phagophore, which originates from specific ER domains, omegasomes, which are enriched in phosphatidylinositol 3-phosphate. Omegasomes facilitate phagophore assembly by recruiting proteins like DFCP1/ZFYVE1...

The study of stop codon reassignment in organisms with alternative genetic codes provides further insight into the evolution of the genetic code and sheds light on translational mechanisms. In eukaryotes, interactions between several factors such as tRNA, eRF1, stop codon context, PABP, and 3′UTR length and structure may determine its ambiguity. ...

This review discusses the role of gene regulatory networks in enhancing understanding of transcriptional regulation and construction methods ranging from expression-based approaches to supervised machine learning. We explore experimental methods, such as MPRAs and CRISPR-Cas9-based screening, and analyze the potential of machine learning and artificial...

BioEssays, EarlyView.

Trypanosoma brucei acquires host macromolecular nutrients such as transferrin (Tf) and haptoglobin-hemoglobin (Hp-Hb) for their survival. This process is mediated by specific receptors embedded within VSG coat, which is constantly at risk of being recognized by host anti-receptor antibodies. We discuss how trypanosome have evolved strategies to overcome...

The Flavin-containing Monooxygenase, fmo-2, in C. elegans is upregulated under various conditions to promote longevity and enhance stress tolerance. We hypothesize that FMO-2-mediated tryptophan metabolism elevates formate levels, consequently modifying the flux of one-carbon metabolism (OCM) and influencing signaling pathways such as mTOR and AMPK,...